Product Info Summary
SKU: | PB10018 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-ZWINT Antibody Picoband™
SKU/Catalog Number
PB10018
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ZWINT Antibody Picoband™ catalog # PB10018. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ZWINT Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10018)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ZWINT recombinant protein (Position: Q29-Q249). Human ZWINT shares 58% and 57.1% amino acid (aa) sequence identity with mouse and rat ZWINT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB10018 is reactive to ZWINT in Human, Mouse, Rat
Applications
PB10018 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
31 kDa
Calculated molecular weight
31.293kDa
Background of ZWINT
ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ZWINT using anti-ZWINT antibody (PB10018).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ZWINT antigen affinity purified polyclonal antibody (Catalog # PB10018) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ZWINT at approximately 31 kDa. The expected band size for ZWINT is at 31 kDa.
Click image to see more details
Figure 2. IHC analysis of ZWINT using anti-ZWINT antibody (PB10018).
ZWINT was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZWINT Antibody (PB10018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ZWINT using anti-ZWINT antibody (PB10018).
ZWINT was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-ZWINT Antibody (PB10018) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For ZWINT (Source: Uniprot.org, NCBI)
Gene Name
ZWINT
Full Name
ZW10 interactor
Weight
31.293kDa
Alternative Names
C20orf164; chromosome 20 open reading frame 164; dJ337O18.7; zinc finger SWIM domain-containing protein 3; zinc finger, SWIM domain containing 3; zinc finger, SWIM-type containing 3 ZWINT HZwint-1, KNTC2AP, SIP301, ZWINT ZW10 interacting kinetochore protein ZW10 interactor|SNAP25 interacting protein of 30 kDa|ZW10 interactor, kinetochore protein|human ZW10 interacting protein-1|zwint-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ZWINT, check out the ZWINT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ZWINT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ZWINT Antibody Picoband™ (PB10018)
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No publications found for PB10018
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-ZWINT Antibody Picoband™
Question
Do you have a BSA free version of anti-ZWINT antibody PB10018 available?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ZWINT antibody PB10018 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-12
Question
Will anti-ZWINT antibody PB10018 work on pig WB with colon?
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
Our lab technicians have not validated anti-ZWINT antibody PB10018 on pig. You can run a BLAST between pig and the immunogen sequence of anti-ZWINT antibody PB10018 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig colon in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-22
Question
I am interested in to test anti-ZWINT antibody PB10018 on rat colon for research purposes, then I may be interested in using anti-ZWINT antibody PB10018 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-27
Answer
The products we sell, including anti-ZWINT antibody PB10018, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-27
Question
We are currently using anti-ZWINT antibody PB10018 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-25
Answer
The anti-ZWINT antibody (PB10018) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-25